
Integrated Sensor Technology for Enhanced Ethanol Monitoring in S. cerevisiae Cultivation.

Project Overview
In industrial processes, microorganisms like Saccharomyces cerevisiae play a key role in producing generic biomass or specific substances such as enzymes, amino acids, and antibiotics. When targeting high biomass yields, S. cerevisiae relies on oxidative metabolism, utilizing glucose for aerobic respiration. However, surpassing a critical glucose concentration triggers ethanol production, known as the Crabtree effect, leading to an undesirable decrease in biomass yield.
To overcome this challenge and optimize productivity, continuous real-time monitoring of ethanol concentration becomes crucial. Currently, there is a notable absence of accurate online methods for measuring ethanol concentrations during yeast cultivation. In response to this gap, our project aims to design and implement an online monitoring tool specifically crafted for real-time ethanol measurements.
Innovative Solution
Our approach combines advanced sensor technology with a model-based calibration strategy. This synergy results in a sophisticated and real-time monitoring tool that ensures precision in assessing ethanol levels.
The core of our innovation lies in the implementation of a gas sensor array and headspace sampling system. This intricately designed setup allows for the accurate prediction of ethanol concentration in the liquid phase during the cultivation process. The gas sensor array captures nuanced data, while the headspace sampling system ensures the representation of the fermentation environment.
Complementing this hardware innovation is our Model-Based Calibration (MBC) Algorithm. Departing from conventional methods, we eliminate the need for offline measurements. Instead, simulated process variables are employed to determine the parameters of the chemometric model. Noteworthy is the fact that our algorithm discerns the initially unknown kinetic parameters of the process model during this calibration procedure.

Efficient Implementation and Impact
Our project successfully introduced a novel method for predicting ethanol concentrations in yeast cultivation, emphasizing the use of a gas sensor array and model-based calibration. The results indicated high accuracy and efficiency, making this approach a cost-effective alternative to traditional monitoring methods. The innovative nature of our solution opens up new possibilities for real-time monitoring in similar processes, showcasing the potential impact on industrial applications and research in yeast cultivation.
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